تخلیص نسبی آنزیم رادانیز (rhodanese)

Reversible Folding of Rhodanese

For the first time completely reversible unfolding was achieved for guanidinium chloride-denatured rhodanese using a systematically defined protocol. These conditions included fl-mercaptoethanol, lauryl maltoside, and sodium thiosulfate. All components were required to get more than the previous best reactivation with lauryl maltoside of 17% (Tandon, S., and Horowitz, P. (1986) J. Biol. Chem. 2...

تخلیص و بررسی فعالیت پروتئولیتیکی آنزیم فیسین انجیر (فیکوس کاریکا )

Introduction & Objective: Ficin is a member of plant cystein proteases that is abundant in fig. This enzyme has many pharmacological and industrial uses. In the present study, the enzyme was purified by a simple procedure and its proteolytic activity was assayed on several plant and animal proteins. Materials & Methods: Ficin was extracted from unripe fig, precipitated by ammonium sulfate and...

Crystalline beef kidney rhodanese.

Although rhodanese activity occurs in many tissues (l-3), the enzyme had been obtained in crystalline form from beef liver only (4). Beef kidney rhodanese could not be crystallized by the same procedure (5). To extend the biochemical genetic comparison reported previously (5), however, it was desired to obtain the kidney enzyme in a very high state of purity. This paper reports the purification...

The rhodanese/Cdc25 phosphatase superfamily

Rhodanese domains are ubiquitous structural modules occurring in the three major evolutionary phyla. They are found as tandem repeats, with the C-terminal domain hosting the properly structured active-site Cys residue, as single domain proteins or in combination with distinct protein domains. An increasing number of reports indicate that rhodanese modules are versatile sulfur carriers that have...

تولید و تخلیص نسبی Streptavidin از محیط کشت باکتری توسط کروماتوگرافی تعویض یونی بوسیله DEAE - سلولز

تخلیص نسبی آنزیم آلژینات لیاز تولید شده از یک سویه موکوئیدی باکتری pseudomonas aeruginosa جداشده از بیمار

باکتری pseudomonas aeruginosa، یک باکتری گرم منفی، هوازی و میله ای شکل متعلق به خانواده pseudomonadaceae می باشد.. این باکتری به دلیل توانایی بالا در تولید پوشش موکوئیدی حاوی اگزوپلی ساکاریدی به نام آلژینات، عامل اصلی مزمن شدن عفونت مجاری تنفسی و در نتیجه مرگ و میر در بیماران مبتلا به فیبروز سیستیک به شمار می آید. یکی از آنزیم های مهم در تولید و تجزیه آلژینات، آلژینات لیاز (algl) نامیده می شود....